Beneficial effects of polysaccharides on "two-hit" acute lung injury and endotoxic fever in rats associated with anti-complementary activities

Thunb. is a traditional herb used for clearing heat and eliminating toxins, and has also been used for the treatment of severe acute respiratory syndrome (SARS). the crude polysaccharides (CHCP) exhibited potent anti-complementary activity through both the classical and alternative pathways by acting on components C3 and C4 of the complement system without interfering with the coagulation system. This study was to investigate the preventive effects of CHCP on acute lung injury (ALI) induced by hemorrhagic shock plus lipopolysaccharide (LPS) instillation (two-hit) and LPS-induced fever in rats. CHCP significantly attenuated pulmonary injury in the "two-hit" ALI model by reducing pulmonary edema and protein exudation in bronchoalveolar lavage fluid (BALF). In addition, it reduced the deposit of complement activation products in the lung and improved oxidant-antioxidant imbalance. Moreover, CHCP administration inhibited fever in rats, reduced the number of leukocytes and restored serum complement levels. The inhibition on the inappropriate activation of complement system by CHCP may play an important role in its beneficial effects on inflammatory diseases. The anti-complementary polysaccharides are likely to be among the key substances for the heat-clearing function of .

Effect observation of float needle combined with reperfusion activity for pain induced by cyclomastopathy / 中国针灸

<p><b>OBJECTIVE</b>To observe the clinical effect of float needle therapy combined with reperfusion activity for pain induced by cyclomastopathy.</p><p><b>METHODS</b>Thirty-five female patients with cyclomastopathy were randomly divided into a comprehensive group (18 cases) and a float needle group (17 cases). In the comprehensive group, float needle manipulation was used at the centre of the biceps brachii belly or the ligature between the affected nipple and breast nodule, about 4 cm beside the exterior margin of the breast, and the reperfusion activity of the affected upper limb and breast was combined. In the float needle group, simple float needle therapy was adopted. In the two groups, treatment was started 7 ± 3 days before menstruation, once every other day. After 3-time treatment, the changes of short form McGill Pain Questionnaire (SF-MPQ) scores before and after treatment, the time of pain relieved during the first treatment, recurrence in 3 months after treatment and the adverse reaction were observed. Also, the clinical effects of the two groups were compared.</p><p><b>RESULTS</b>Immediately at the end of the first treatment,after 3-time treatment and while followed up for one month, each item score and the total score of SF-MPQ were decreased apparently than the scores before treatment (all P < 0.05), and all the scores mentioned above of the comprehensive group were declined more obviously than those of the float needle group (all P < 0.05). The time of pain relieved during the first treatment of the comprehensive group was much shorter than that of the float needle group [(94.72 ± 33.67) s vs (162.06 ± 29.16) s, P < 0.01]. The recurrence rate of the comprehensive group in 3 months after treatment was 5.9% (1/17), which was better than 20.0% (3/15) of the float needle group (P < 0.01).</p><p><b>CONCLUSION</b>Float needle therapy combined with reperfusion activity and simple float needle therapy can both safely and effectively improve cyclomastopathy, and the combination therapy is better than simple float needle therapy in the aspects of pain relieving effect at once and the long term effect.</p>

Experimental training of cell immunology based on pathogen-induced diseases——Teaching reformation on experimental courses of medical immunology / 中华医学教育探索杂志

To accommodate the requirement of quality education and achieve the goal of the 7-year-schooling clinical medicine,experimental training of cell immunology based on pathogen-induced immunology was established in combination with traditional experiment teaching method.In this course,inductive teaching method was used.The independent study and innovation ability of student were trained,which had a better teaching effect.

Experimental model of post-operative intra-peritoneal adhesion in rats / 医学研究生学报

Objective:To set up an animal model of intraabdominal adhesion after surgery. Methods:A new rat model of intraabdominal adhesion was made by using pincers,dry gauze,hemostatic clamps and razor blade.Rat appendix was used to produce intestinal adhesions.Forty male Sprague-Dawley rats were divided into 4 groups: group A(gauze group),group B(hemostatic clamp group),group C(pincer group),group D(razor blade group),10 rats in each group. Animals were fed for 7 days before killed.The extent of adhesion was evaluated by Phillips scale and analyzed statistically. Results: The extents of adhesion in group A and B were grade Ⅰ by Phillips scale,grade IV in group C,grade Ⅲ in group D.The extent of adhesion in group C was different significantly from group A,B,and D(P